Arsenic in Rice Group Project:





Arsenic in Rice Group Project:
-       Can be caused by pesticides, fertilizers, water, soil
-       Arsenic Test Kit
-       Climate needs: Warm wet weather
-       Arsenic can be found in water

     Problem Definition/ Literature Review (5 points, MJ)
-       Thousands of metric tons of rice are consumed each year by millions of people worldwide. This is an important source of nutrition for many countries around the world, especially Asia. This is also a massive problem our world faces today because arsenic is found in the consumed rice which is very dangerous for humans to ingest and has been linked to numerous diseases. We believe the way the rice is grown may be the underlying reason arsenic is found in it. Researchers are interested to find out the causes of high arsenic and ways to eliminate this health hazard (SOHN, 2014). Arsenic levels have been rising in rice every year, which makes this issue time sensitive (Editorial, 2016). We will test this rice and the elements used to grow the rice to understand how the arsenic infiltrates the rice and eventually our bodies. Then, we can begin a new way of growing rice safely for everyone.

     Current Situation, where are we now (5 points MJ)
-       The arsenic levels are rising in rice, this is a dangerous element found in rice. The long-term effects of arsenic are devastating for human health. We want to study the environment which grows the rice with high arsenic levels, mainly found in brown rice. In the study, we will block for multiple confounding variables to isolate where we believe the rice will be found. We will test brown rice because it has the highest levels of arsenic and therefore will be the easiest and most helpful to see change in. We will test four states which have been known to have the highest arsenic levels i.e. Arkansas, Missouri, Texas, and Louisiana (https://dailyhealthpost.com/arsenic-in-rice/). and long-term effects of arsenic are considered. The researchers tested water and soil of each location rice was grown.


     Design of Experiment (Davis, 40 points):

1.)   Test all the rice and blocking for confounding variables; Fertilizers and locations.
-       Chose Texas, Arkansas, Missouri, and Louisiana because these states produce the most arsenic in their rice compared to the rest of America.
-       We will have 40 rice plants per block
-       We are only testing brown rice because the highest levels are arsenic are found in brown[1] . Therefore, brown should be the easiest to see change in and will be the most helpful to the world to solve first. We also would like to keep the cost of the study as low as possible. So, we would like to stay in the United States.
-       We will also block by fertilizer vs no fertilizer so we will know if fertilizer is helping or hurting the cultivation of clean healthy rice. It is known that Arsenic can be found in animal food which is then deposited by the animal into the soil. Therefore, a better fertilizer may be our answer.


2.)    After this test is complete, we will test the growing conditions of the rice to find if   the arsenic is due to other environmental factors such as soil or water. Essentially, we will study the resources that the plant uses to grow itself. Then we can compare chemical levels of the rice to the resources it used to grow. This way we will know if the arsenic levels are in the rice itself or the plants food.
-       Collect rainwater samples each day it rains throughout growing cycle
-       Collect soil samples each day throughout growing cycle
-       Send samples to lab to be evaluated for arsenic levels
3.)   Once the step above is complete we will evaluate the data. Depending on where we find the arsenic after the tests. Whether it’s; Water, soil, location, or fertilizer. We will retest with a higher emphasis on the conflicting variable found.

     Comparing Methods (20 points, Jake)

In this sample, we are going to use a two factor ANOVA test. A z-test will also be utilized because we have more than thirty samples.

We decided against the t-test because a t-test utilizes only two groups and our test has eight groups.



“While there is no federal standard for arsenic in food, according to the Consumers Union, the advocacy arm of Consumer Reports, one serving of rice may have as much inorganic arsenic as an entire day’s worth of water. “-
^^^this article has a lot of good shit

1)    First, the rice will be assessed to see which states produce the highest arsenic levels

2)    A preliminary background of lead-arsenate pesticide use should be tracked from past usage
Arsenic in rice can also be due to animal waste from hogs and chickens that consume food containing arsenic feed (used to make animals grow faster)

3)    Conduct tests to assess whether pesticide use and/or arsenic feed impacts the levels of arsenic found in participating farmlands (state average as a whole)

4)    Test the air, rain, and soil (earth’s crust also?) of the three highest rice producing farms in each state

5)    Draw the two states with the highest arsenic levels for comparison with one another


6)    Next, the rice will be assessed in the categories of white and brown rice
The brown rice typically has higher arsenic levels, however, test the variance of arsenic levels in each type of rice

            ^ I did this section wrong, but I am leaving this info in case it finds itself useful



     Plans for completion (5 points, Ahmed)
-       In this project we are supposed to design an experiment which will include research and analysis about the current growing conditions of rice produced in the United States. We chose four states, Arkansas, Missouri, Texas, and Louisiana where they considered as significant sources of distributing rice around the United States. In addition, we selected to study the brown rice because it is known for having high arsenic levels. We found that water, soil, fertilizers and the animals in the selected lands could play as root causes in rising the arsenic level according to the research we did. As a result, after collecting data and performing the tests, we would be able to identify the root causes and be able to fix the problems.       

-       This project is estimated to take approximately six months to complete and samples should be collected during the growing season of rice. Rice is grown in warm weather and generally planted in the spring while taking three to six months to grow. Because of this a start time around the two-three months into growing would be ideal, this would fall around May. Each farm will need to be traveled to in order to collect the samples then there will be a waiting period to get the data back once the rice samples are sent in. Once that is completed the data will be analyzed, this is estimated to take roughly three months. Once we are aware of which farms require further analysis the environmental samples will be sent to a lab for analysis. The return and analysis of the environmental data is estimated to take another three months totaling the estimated six-month completion for the project.
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     Scope/Budget (5 points, Ahmed)
-       In this experiment, we aimed to collect data form such multiple state and such several farms from each state which considered as big sources of distributing rice around the United States. The experiment will focus on the brown since the research shows that it has a higher arsenic level. We used the blocking method to specify the confounding factors; locations and fertilizers. The test will block the rice plants by groups of location, then block by fertilization treatments against no without fertilization treatments. We will design the group to have 40 samples of rice plants per each block at the end. The project will include the testing of the growing conditions of planting the brown rice. We think that water and soil can play a role in our experiment. As a result, we are going to collect samples of rainwater and soil to test the arsenic level in them. Then, we will analyze the results found on the test. However, we are going to ignore the air as confounding factor since arsenic is barely exists in the air.  In addition, this project will introduce the comparing methods between each group using two factor ANOVA test and z-test. Later, the group will perform analyze the effect of adding purified water vs. rainwater and study the history of animals on the chosen lands. Moreover, the project will show the results of test performed on the types of fertilizers in which we randomly chose.
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     Analysis and next steps (10 points, Davis)
-       If arsenic is found in the water and nowhere else, we will run an experiment using purified water that is pretested to have zero arsenic in it.
-       Then, retest the rice afterwards to verify if we solved the issue at hand



-       If we find the arsenic only in one state and believe it has to do with the states and growing conditions, we will do a retest growing brown rice in the

-       If the arsenic is found to be in the soil or fertilizer will test the top 3 fertilizers and 1 natural compost, and a control group using regular dirt.


-       If we find arsenic in all samples tested, we will test the seeds from suppliers we used vs seeds of the top 3 rice seed cultivators. We will send them to a lab to test pre growth arsenic levels to find out whether the arsenic starts from the very beginning of the rice growing process.


-       If we find arsenic nowhere in these rice samples then we will retest using a larger sample size of 100 and using more climates around the world.

     Lesson Learned

       ​This project will allow an understanding how much the arsenic is negatively impacting the health of human as millions of people eat rice on daily basis. The increased level of arsenic in rice can cause a lot of issues for the people as this is difficult to digest and leads towards health issues. In Asian countries, rice is a source of nutrition, people cannot quit eating rice. This project allows analyzing that arsenic ratio in the rice increased while the rice is grown, and it is commonly found in the brown rice. I have learned that it is important to reduce the level of arsenic in rice so that the threat to human health can be reduced.

         ​Another learning from this project is, arsenic has a negative impact on the health which are devasting. We have decided to study the environment where the rice is grown, brown rice will be tested as arsenic level is quite high in this type of rice. We decided to work in the specific areas, for instance Texas, Louisiana, Missouri and Arkansas. Water and soil of each location are tested to check the level of arsenic in the rice grown in these areas.




Bibliography
Drug, U. F. (2016). FDA Statement on Testing and Analysis of Arsenic in Rice and Rice Products. Retrieved from https://www.fda.gov/food/metals/fda-statement-testing-and-analysis-arsenic-rice-and-rice-products
Editorial, D. (2016). Store-Bought Rice is Filled with Arsenic…Here’s How to Remove as Much as Possible! Retrieved from https://dailyhealthpost.com/arsenic-in-rice/
SOHN, E. (2014). The toxic side of rice. Nature .





Whoever found this info can you post the link and cite it. idk where it is but I remember us talking about it.
I found that arsenic can be in the air